Polymerase Chain Reaction:Article Title: Protein kinase Cδ is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria
Article Snippet: .. 72116 16 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554,714 Commercial assay or kit QIAquick PCR Purification Kit QIAGEN Cat# 28,106 Commercial assay or kit RNeasy Micro QIAGEN Cat# 74,004 Commercial assay or kit Thunderbird SYBR qPCR Mix TOYOBO Cat# QPS- 201 Commercial assay or kit SuperPrep II Cell lysis & RT Kit for qPCR TOYOBO Cat# SCQ- 401 Commercial assay or kit CellTraceTM Violet (CTV) Cell proliferation Kit Thermo Fisher Scientific Cat# C34557 Chemical compound, drug PPS3 ATCC Cat# 169 X Chemical compound, drug LPS Sigma Cat# L2880 Chemical compound, drug R- 848 InvivoGen Cat# tlrl- r848 Chemical compound, drug CpG ODN 1826 InvivoGen Cat# tlrl- 1826 Chemical compound, drug Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65- 0866- 18 Chemical compound, drug DOTAP Liposomal Transfection Reagent Sigma Cat# 11202375001 Chemical compound, drug TRI Reagent Sigma Cat# T9424 Chemical compound, drug PEI Max (Mw 40,000) Polysciences Cat# 24765- 1 Chemical compound, drug ReverTra Ace TOYOBO Cat# TRT- 101 Chemical compound, drug KOD Fx Neo DNA polymerase TOYOBO Cat# KFX- 201 Chemical compound, drug [3 H] thymidine PerkinElmer Cat# NET027001MC Chemical compound, drug anti- APC MicroBeads MicroBeads Cat# 130- 090- 855 Chemical compound, drug GoTaq Green Master Mix Promega Cat# M712B Chemical compound, drug DSS MP Biomedicals Cat# 9011- 18- 1 Chemical compound, drug Brain Heart Infusion Agar BD Bioscience Cat# 221,570 Software, algorithm FlowJo https://www. flowjo. com/ solutions/flowjo RRID:SCR_008520 Software, algorithm GraphPad Prism https://graphpad. com RRID:SCR_002798 Continued Fukao et al. eLife 2021;10:e72116. ..
Purification:Article Title: Protein kinase Cδ is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria
Article Snippet: .. 72116 16 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554,714 Commercial assay or kit QIAquick PCR Purification Kit QIAGEN Cat# 28,106 Commercial assay or kit RNeasy Micro QIAGEN Cat# 74,004 Commercial assay or kit Thunderbird SYBR qPCR Mix TOYOBO Cat# QPS- 201 Commercial assay or kit SuperPrep II Cell lysis & RT Kit for qPCR TOYOBO Cat# SCQ- 401 Commercial assay or kit CellTraceTM Violet (CTV) Cell proliferation Kit Thermo Fisher Scientific Cat# C34557 Chemical compound, drug PPS3 ATCC Cat# 169 X Chemical compound, drug LPS Sigma Cat# L2880 Chemical compound, drug R- 848 InvivoGen Cat# tlrl- r848 Chemical compound, drug CpG ODN 1826 InvivoGen Cat# tlrl- 1826 Chemical compound, drug Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65- 0866- 18 Chemical compound, drug DOTAP Liposomal Transfection Reagent Sigma Cat# 11202375001 Chemical compound, drug TRI Reagent Sigma Cat# T9424 Chemical compound, drug PEI Max (Mw 40,000) Polysciences Cat# 24765- 1 Chemical compound, drug ReverTra Ace TOYOBO Cat# TRT- 101 Chemical compound, drug KOD Fx Neo DNA polymerase TOYOBO Cat# KFX- 201 Chemical compound, drug [3 H] thymidine PerkinElmer Cat# NET027001MC Chemical compound, drug anti- APC MicroBeads MicroBeads Cat# 130- 090- 855 Chemical compound, drug GoTaq Green Master Mix Promega Cat# M712B Chemical compound, drug DSS MP Biomedicals Cat# 9011- 18- 1 Chemical compound, drug Brain Heart Infusion Agar BD Bioscience Cat# 221,570 Software, algorithm FlowJo https://www. flowjo. com/ solutions/flowjo RRID:SCR_008520 Software, algorithm GraphPad Prism https://graphpad. com RRID:SCR_002798 Continued Fukao et al. eLife 2021;10:e72116. ..
Real-time Polymerase Chain Reaction:Article Title: Protein kinase Cδ is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria
Article Snippet: .. 72116 16 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554,714 Commercial assay or kit QIAquick PCR Purification Kit QIAGEN Cat# 28,106 Commercial assay or kit RNeasy Micro QIAGEN Cat# 74,004 Commercial assay or kit Thunderbird SYBR qPCR Mix TOYOBO Cat# QPS- 201 Commercial assay or kit SuperPrep II Cell lysis & RT Kit for qPCR TOYOBO Cat# SCQ- 401 Commercial assay or kit CellTraceTM Violet (CTV) Cell proliferation Kit Thermo Fisher Scientific Cat# C34557 Chemical compound, drug PPS3 ATCC Cat# 169 X Chemical compound, drug LPS Sigma Cat# L2880 Chemical compound, drug R- 848 InvivoGen Cat# tlrl- r848 Chemical compound, drug CpG ODN 1826 InvivoGen Cat# tlrl- 1826 Chemical compound, drug Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65- 0866- 18 Chemical compound, drug DOTAP Liposomal Transfection Reagent Sigma Cat# 11202375001 Chemical compound, drug TRI Reagent Sigma Cat# T9424 Chemical compound, drug PEI Max (Mw 40,000) Polysciences Cat# 24765- 1 Chemical compound, drug ReverTra Ace TOYOBO Cat# TRT- 101 Chemical compound, drug KOD Fx Neo DNA polymerase TOYOBO Cat# KFX- 201 Chemical compound, drug [3 H] thymidine PerkinElmer Cat# NET027001MC Chemical compound, drug anti- APC MicroBeads MicroBeads Cat# 130- 090- 855 Chemical compound, drug GoTaq Green Master Mix Promega Cat# M712B Chemical compound, drug DSS MP Biomedicals Cat# 9011- 18- 1 Chemical compound, drug Brain Heart Infusion Agar BD Bioscience Cat# 221,570 Software, algorithm FlowJo https://www. flowjo. com/ solutions/flowjo RRID:SCR_008520 Software, algorithm GraphPad Prism https://graphpad. com RRID:SCR_002798 Continued Fukao et al. eLife 2021;10:e72116. ..
Lysis:Article Title: Protein kinase Cδ is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria
Article Snippet: .. 72116 16 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554,714 Commercial assay or kit QIAquick PCR Purification Kit QIAGEN Cat# 28,106 Commercial assay or kit RNeasy Micro QIAGEN Cat# 74,004 Commercial assay or kit Thunderbird SYBR qPCR Mix TOYOBO Cat# QPS- 201 Commercial assay or kit SuperPrep II Cell lysis & RT Kit for qPCR TOYOBO Cat# SCQ- 401 Commercial assay or kit CellTraceTM Violet (CTV) Cell proliferation Kit Thermo Fisher Scientific Cat# C34557 Chemical compound, drug PPS3 ATCC Cat# 169 X Chemical compound, drug LPS Sigma Cat# L2880 Chemical compound, drug R- 848 InvivoGen Cat# tlrl- r848 Chemical compound, drug CpG ODN 1826 InvivoGen Cat# tlrl- 1826 Chemical compound, drug Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65- 0866- 18 Chemical compound, drug DOTAP Liposomal Transfection Reagent Sigma Cat# 11202375001 Chemical compound, drug TRI Reagent Sigma Cat# T9424 Chemical compound, drug PEI Max (Mw 40,000) Polysciences Cat# 24765- 1 Chemical compound, drug ReverTra Ace TOYOBO Cat# TRT- 101 Chemical compound, drug KOD Fx Neo DNA polymerase TOYOBO Cat# KFX- 201 Chemical compound, drug [3 H] thymidine PerkinElmer Cat# NET027001MC Chemical compound, drug anti- APC MicroBeads MicroBeads Cat# 130- 090- 855 Chemical compound, drug GoTaq Green Master Mix Promega Cat# M712B Chemical compound, drug DSS MP Biomedicals Cat# 9011- 18- 1 Chemical compound, drug Brain Heart Infusion Agar BD Bioscience Cat# 221,570 Software, algorithm FlowJo https://www. flowjo. com/ solutions/flowjo RRID:SCR_008520 Software, algorithm GraphPad Prism https://graphpad. com RRID:SCR_002798 Continued Fukao et al. eLife 2021;10:e72116. ..
Transfection:Article Title: Protein kinase Cδ is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria
Article Snippet: .. 72116 16 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554,714 Commercial assay or kit QIAquick PCR Purification Kit QIAGEN Cat# 28,106 Commercial assay or kit RNeasy Micro QIAGEN Cat# 74,004 Commercial assay or kit Thunderbird SYBR qPCR Mix TOYOBO Cat# QPS- 201 Commercial assay or kit SuperPrep II Cell lysis & RT Kit for qPCR TOYOBO Cat# SCQ- 401 Commercial assay or kit CellTraceTM Violet (CTV) Cell proliferation Kit Thermo Fisher Scientific Cat# C34557 Chemical compound, drug PPS3 ATCC Cat# 169 X Chemical compound, drug LPS Sigma Cat# L2880 Chemical compound, drug R- 848 InvivoGen Cat# tlrl- r848 Chemical compound, drug CpG ODN 1826 InvivoGen Cat# tlrl- 1826 Chemical compound, drug Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65- 0866- 18 Chemical compound, drug DOTAP Liposomal Transfection Reagent Sigma Cat# 11202375001 Chemical compound, drug TRI Reagent Sigma Cat# T9424 Chemical compound, drug PEI Max (Mw 40,000) Polysciences Cat# 24765- 1 Chemical compound, drug ReverTra Ace TOYOBO Cat# TRT- 101 Chemical compound, drug KOD Fx Neo DNA polymerase TOYOBO Cat# KFX- 201 Chemical compound, drug [3 H] thymidine PerkinElmer Cat# NET027001MC Chemical compound, drug anti- APC MicroBeads MicroBeads Cat# 130- 090- 855 Chemical compound, drug GoTaq Green Master Mix Promega Cat# M712B Chemical compound, drug DSS MP Biomedicals Cat# 9011- 18- 1 Chemical compound, drug Brain Heart Infusion Agar BD Bioscience Cat# 221,570 Software, algorithm FlowJo https://www. flowjo. com/ solutions/flowjo RRID:SCR_008520 Software, algorithm GraphPad Prism https://graphpad. com RRID:SCR_002798 Continued Fukao et al. eLife 2021;10:e72116. ..
Software:Article Title: Protein kinase Cδ is essential for the IgG response against T-cell-independent type 2 antigens and commensal bacteria
Article Snippet: .. 72116 16 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Commercial assay or kit Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554,714 Commercial assay or kit QIAquick PCR Purification Kit QIAGEN Cat# 28,106 Commercial assay or kit RNeasy Micro QIAGEN Cat# 74,004 Commercial assay or kit Thunderbird SYBR qPCR Mix TOYOBO Cat# QPS- 201 Commercial assay or kit SuperPrep II Cell lysis & RT Kit for qPCR TOYOBO Cat# SCQ- 401 Commercial assay or kit CellTraceTM Violet (CTV) Cell proliferation Kit Thermo Fisher Scientific Cat# C34557 Chemical compound, drug PPS3 ATCC Cat# 169 X Chemical compound, drug LPS Sigma Cat# L2880 Chemical compound, drug R- 848 InvivoGen Cat# tlrl- r848 Chemical compound, drug CpG ODN 1826 InvivoGen Cat# tlrl- 1826 Chemical compound, drug Fixable Viability Dye eFluor 506 Thermo Fisher Scientific Cat# 65- 0866- 18 Chemical compound, drug DOTAP Liposomal Transfection Reagent Sigma Cat# 11202375001 Chemical compound, drug TRI Reagent Sigma Cat# T9424 Chemical compound, drug PEI Max (Mw 40,000) Polysciences Cat# 24765- 1 Chemical compound, drug ReverTra Ace TOYOBO Cat# TRT- 101 Chemical compound, drug KOD Fx Neo DNA polymerase TOYOBO Cat# KFX- 201 Chemical compound, drug [3 H] thymidine PerkinElmer Cat# NET027001MC Chemical compound, drug anti- APC MicroBeads MicroBeads Cat# 130- 090- 855 Chemical compound, drug GoTaq Green Master Mix Promega Cat# M712B Chemical compound, drug DSS MP Biomedicals Cat# 9011- 18- 1 Chemical compound, drug Brain Heart Infusion Agar BD Bioscience Cat# 221,570 Software, algorithm FlowJo https://www. flowjo. com/ solutions/flowjo RRID:SCR_008520 Software, algorithm GraphPad Prism https://graphpad. com RRID:SCR_002798 Continued Fukao et al. eLife 2021;10:e72116. ..
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